GLOW (TB-500/BPC-157/GHK-CU (10mg/5mg/25mg)

Description

Product Overview:

Glow Blend by Peptide Technologies is a high-purity, lyophilized peptide blend formulated for research use only. It combines three distinct peptides – BPC 157 (5 mg), TB 500 (5 mg), and GHK-Cu (50 mg) – in a stabilized matrix containing mannitol as a bulking agent. Each peptide is provided in its synthetic form with precise chemical characteristics, making Glow Blend suitable for analytical and laboratory applications. This product is not intended for human or veterinary use.

Composition and Key Properties

• BPC 157 (Pentadecapeptide) – 5 mg
  • Sequence: Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val (15 amino acids).
  • Molecular Formula: C₆₂H₉₈N₁₆O₂₂.
  • Molecular Weight: Approximately 1,419.5 Da (free base form).
  • CAS Number: 137525-51-0.
  • Peptide Profile: Also known as Body Protection Compound-157, this gastric pentadecapeptide fragment is synthetically derived. It is provided as a white to off-white lyophilized powder (often as an acetate salt). The amino acid sequence corresponds to a naturally occurring protective protein fragment.
• TB 500 (Thymosin Beta-4 Fragment) – 5 mg
  • Sequence: N-Acetyl-Leu-Lys-Lys-Thr-Glu-Thr-Gln (Ac-LKKTETQ; 7 amino acids).
  • Molecular Formula: C₃₈H₆₈N₁₀O₁₄ (for the acetylated heptapeptide).
  • Molecular Weight: Approximately 889.0 Da (free base).
  • CAS Number: 885340-08-9.
  • Peptide Profile: TB 500 is a synthetic peptide corresponding to the active region (residues 17–23) of Thymosin β4. It is an acetylated fragment (acetate salt) provided as a stable white lyophilized powder. This fragment’s sequence (LKKTETQ) is known for its role in various cellular processes, but no medical or therapeutic claims are made for this research product.
• GHK-Cu (Copper Tripeptide-1) – 50 mg
  • Sequence: Gly-His-Lys • Cu^2+ (tripeptide complex with copper)​
  • Molecular Formula: C₁₄H₂₂CuN₆O₄ (for the copper-bound tripeptide)​
  • Molecular Weight: Approximately 401.9 Da (copper complex)​
  • CAS Number: 89030-95-5 (commonly referenced for the GHK-Cu complex)​
  • Peptide Profile: GHK-Cu is a tripeptide (glycyl-L-histidyl-L-lysine) that has formed a 1:1 complex with a divalent copper ion​
    In this blend, it is present as a blue-tinged lyophilized powder due to the copper content. The peptide itself (GHK) has a formula C₁₄H₂₄N₆O₄ (molecular weight ~340.4 Da), and binding to Cu^2+ adds to the formula weight. The GHK-Cu is included at a higher quantity (50 mg) to ensure adequate availability for research assays requiring the copper peptide.

Chemical Information

Amino Acid Sequences and Profiles:

• BPC 157: Contains glycine (Gly), glutamic acid (Glu), proline (Pro) – repeated, lysine (Lys), alanine (Ala), aspartic acid (Asp), leucine (Leu), and valine (Val). Its sequence is denoted as GEPPPGKPADDAGLV, corresponding to the single-letter amino acid code. It is a pentadecapeptide (15-mer) with an N-terminal Gly and C-terminal Val (carboxylate). The IUPAC name and structure identify a linear peptide chain (no disulfide bonds). PubChem lists the molecular formula and computed molecular weight (~1419.5 g/mol) for the base peptide. BPC 157 is typically provided as an acetate salt in research materials, which can slightly increase the net weight (e.g., trifluoroacetate salt MW ~1479.6), but Glow Blend specifies the peptide content by free base weight.
• TB 500: This is a fragment of the larger Thymosin Beta-4 (which is a 43-amino acid protein with MW ~4963 Da). TB 500 specifically refers to the synthetic acetylated fragment Ac-LKKTETQ-OH, which includes an N-terminal acetylated leucine (Ac-Leu) followed by Lys-Lys-Thr-Glu-Thr-Gln and a free C-terminus. The acetylation adds to stability and mimics the natural N-acetylserine of Thymosin β4’s N-terminus (in native TB4, the sequence starts with Ac-Ser-Asp-Lys-Pro-…, but the active site is within the LKKTETQ region). The chemical formula C₃₈H₆₈N₁₀O₁₄ and molecular weight ~889 Da reflect the presence of the acetyl group and the seven amino acid residues. This peptide is often supplied as the acetate salt for stability; however, the formula and weight given are for the peptide itself (excluding counter-ions). The structure is linear with no post-translational modifications other than N-acetylation.
• GHK-Cu: GHK is a tripeptide consisting of glycine, histidine, and lysine (sequence typically written as H-Gly-His-Lys-OH). In Glow Blend, it is present as the copper (II) complex, which is often termed GHK-Cu or Copper Tripeptide-1. The copper is chelated by the tripeptide, forming a coordination complex. The molecular formula for the complex is often noted as C₁₄H₂₂CuN₆O₄ (this represents the deprotonated tripeptide anion plus Cu^2+)​, and the molecular weight is about 401.9 Da​For reference, the free peptide GHK has CAS 49557-75-7 with formula C₁₄H₂₄N₆O₄ and weight ~340.4 Da. The copper complex CAS 89030-95-5​ is often listed in chemical catalogs for the GHK-Cu acetate salt (a common form for cosmetic peptides). The presence of Cu^2+ gives the lyophilized peptide a distinctive light blue color. In solution, GHK readily binds copper ions, forming a stable chelate; this complexation is fully formed in the supplied Glow Blend

Molecular Structures:
(Note: Structural depictions are described for clarity since visual embedding is not available.)

• BPC 157: Linear peptide structure with 15 amino acids. The molecule has multiple peptide bonds connecting each residue in the sequence Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val. The overall structure is a flexible chain with polar side groups (from Glu, Asp, Lys) and hydrophobic groups (from Pro, Leu, Val). The PubChem CID for BPC-157 is 9941957, which provides detailed 2D structure and SMILES notation.
• TB 500: A smaller linear peptide fragment. Its sequence (Ac-LKKTETQ) indicates an acetylated N-terminus and a C-terminal glutamine. The peptide’s side chains include basic groups (two Lysines), polar threonines and glutamine, and a glutamic acid. The absence of cysteine means no disulfide bonds. The compact size (7 residues) and acetylated N-terminus contribute to TB-500’s stability. PubChem lists a substance corresponding to this fragment (UNII-QHK6Z47GTG) with the given formula and a similar CID.
• GHK-Cu: The tripeptide (glycine-histidine-lysine) coordinates a Cu^2+ ion typically through the histidine’s imidazole nitrogen and the deprotonated amide nitrogens of glycine and histidine (creating a square planar or distorted tetrahedral complex). The copper tripeptide-1 complex can be conceptually visualized as the small tripeptide (which itself is C₁₄ backbone with glycine as N-terminal) attached to a central Cu^2+. The presence of copper completes the coordination sphere (often involving water or acetate in crystal forms, though Glow Blend is formulated with mannitol rather than additional counter-ions). The PubChem CID 378611 corresponds to Cu-GHK and confirms the molecular weight ~403.92 for one form of the complex; small variations arise if counter-ions like acetate are included (for example, GHK-Cu acetate includes an extra acetate anion, raising formula to C₁₆H₂₆CuN₆O₆ and weight ~462.0 Da, not the case here).

Formulation and Stability

• Lyophilized Powder: Glow Blend is provided as a freeze-dried (lyophilized) powder. Lyophilization enhances the stability of peptides by removing water and creating a solid matrix that is less prone to degradation. Each vial of Glow Blend contains BPC 157, TB 500, and GHK-Cu in the stated amounts, along with pharmaceutical-grade mannitol as a stabilizer and bulking agent.
• Role of Mannitol: Mannitol is a polyol (sugar alcohol) commonly used in peptide formulations as a cryoprotectant and bulking agent. In lyophilized peptides, mannitol helps stabilize the peptides’ structure during the freeze-drying process and improves the cake consistency for reconstitution. It contributes to the physical integrity of the dried powder and can aid in peptide longevity by diluting potentially reactive peptide-peptide interactions. Mannitol generally remains inert in biochemical assays and dissolves readily in aqueous solutions along with the peptides.
• Storage Conditions: For maximum stability, store Glow Blend vials at -20 °C in a dry environment. Peptides BPC 157 and TB 500, in particular, are known to be stable for long-term storage at -20 °C when lyophilized. GHK-Cu is also stable under these conditions, though it should be protected from prolonged exposure to light (as copper complexes can be photosensitive). Once reconstituted (e.g., in sterile water or buffer), aliquot and freeze any unused portion at -20 °C, and use within a short period (typically 1–2 weeks at 4 °C, or longer at -20 °C). Avoid repeated freeze-thaw cycles to preserve peptide integrity.

Quality and Purity

• Purity: Each peptide component in Glow Blend is synthesized and HPLC-purified to a purity of ≥98% (by area normalization). The blend is formulated by weight, and quality control includes amino acid analysis and mass spectrometry to verify the molecular weight of each peptide component. For instance, mass spectrometry will show a monoisotopic mass around 1418.7 Da for BPC 157 (consistent with its formula), ~888.5 Da for TB 500, and ~401.9 Da for GHK-Cu (when measured as the complex).
• Identification:
  • BPC 157: Verified by amino acid sequencing (15-mer matching GEPPPGKPADDAGLV) and comparison to reference standards. CAS 137525-51-0 identity is confirmed via library match.
  • TB 500: Verified by peptide mapping and MS (observing the acetylated N-terminus fragment). CAS 885340-08-9 corresponds to this sequence, confirmed by reference spectra.
  • GHK-Cu: Verified by its unique UV-visible signature (around 600-800 nm due to the Cu^2+ peptide complex’s slight absorbance) and by MS which shows a parent ion consistent with the copper-bound tripeptide. The free peptide (GHK) can be verified by amino acid analysis (Gly:His:Lys in 1:1:1 ratio), and copper content is confirmed by atomic absorption or ICP-MS.
• Additives: Mannitol (~some milligrams, precise amount proprietary) is the only excipient. There are no other fillers, preservatives, or carriers. The lyophilization process may involve acetic acid (from acetate salts) which, if present, is in trace amounts as residual counter-ions. The product is trifluoroacetate-free (since some peptide syntheses leave TFA salts; Glow Blend uses acetate forms to minimize TFA content).

Handling and Usage

• Usage Instructions: Use appropriate personal protective equipment (gloves, lab coat, eye protection) when handling. Reconstitute the lyophilized peptide blend in a clean environment to avoid contamination. Calculate the final peptide concentrations based on the 5 mg BPC 157 + 5 mg TB 500 + 50 mg GHK-Cu content; for example, reconstituting in 5 mL yields concentrations of 1 mg/mL BPC 157, 1 mg/mL TB 500, and 10 mg/mL GHK-Cu. Always mix well until fully dissolved.
• Research Use Only: Glow Blend is labeled For Research Use Only. It is not a drug, vitamin, or dietary supplement. Any research application should be in vitro or in appropriate laboratory animals, per applicable regulations. No claims are made regarding therapeutic effects or health benefits of this product. It is not intended to diagnose, treat, cure, or prevent any disease.
• Shelf Life: When stored at -20 °C in a desiccated environment, the shelf life is 24 months from the date of manufacture. Each vial is batch-coded for traceability, and a certificate of analysis (COA) is available, detailing peptide content and analytical verification including HPLC purity and MS data.
• Disposal: Dispose of any unused or expired product according to laboratory safety protocols. Because this product contains bioorganic peptides and copper, follow biohazard and heavy metal disposal guidelines if applicable (though the quantities of copper are small, typically in the microgram range per vial). Decontaminate surfaces with standard laboratory disinfectants after handling.

References

To ensure accuracy in the chemical and technical details provided, the following sources were referenced:

• PubChem compound summaries and vendor data for molecular weights, formulas, and sequences of BPC 157, TB 500, and GHK/GHK-Cu​
  particlepeptides.com
• Peptide chemistry catalogs and data sheets confirming amino acid sequences and CAS numbers​
  particlepeptides.com
• Scientific literature on lyophilized peptide formulation and the role of mannitol as a stabilizer (e.g., mannitol’s use as a bulking agent for improved peptide stability).

All data are sourced from established chemical databases or product literature to ensure reliability and traceability of the information presented. The structured format follows best practices for technical product descriptions, facilitating quick reference to key properties for each component of Glow Blend.